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Urea UV-KINETIC

Intended Use:

Test for estimation of Urea in serum / plasma using UV-KINETIC method.

Introduction:

Urea is the end product of protein metabolism. It is synthesized in liver and transported to kidney for excretion. Increased Urea / Urea Nitrogen levels are associated with renal diseases as well as dehydration, gastrointestinal hemorrhage and diabetic coma. Decreased values are observed in some cases of severe liver diseases. Urea can be estimated in serum / plasma using UV-kinetic method.

Features:

Enzymatic Method Specific For Urea
Liquid Stable Reagents With Long Shelf Life
Fast Result
High Linearity
Suitable for Fully Automated Chemistry Analyzer

Specifications

Methodology	Urease-GLDH UV Kinetic
Number Of Reagents	Two
Sample Type	Human Serum or Plasma
Reaction Type	Fixed Time
Reaction Direction	Decreasing
Blank Settings	Water
Blank Absorbance Limit	NA
Primary Wavelength	340 nm
Secondary Wavelength	NA
Flow Cell Temperature	37 0C
R1:R2 Mixing Ratio	4:1
Reagent Volume R1	800 µL
Reagent Volume R2	200 µL
Sample Volume	100 µL
Delay Time	30 Seconds
Measuring Time	120 Seconds
Standard Concentration	50 mg/dl
Incubation Temperature	37 0C
Unit	mg/dl
Linearity	350 mg/dl
Pack Sizes	50 ml –(2×20 ml +2x5 ml ), 100 ml – 4×20 ml + 4×5 ml)
Shelf life	24 months
Storage Temperature	2-8 0C

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