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Total Protein

Total Protein

Intended Use:

Test for estimation of Total protein in serum / plasma using BEIRUT method .

Introduction:

A total protein test measures the total amount of protein in the blood. It also measures the amounts of two major groups of proteins in the blood: albumin and globulin. Increased levels of Total Protein are found in dehydration, multiple myeloma and chronic liver diseases. Decreased levels are found in renal diseases and terminal liver failure. Total Protein can be estimated in serum / plasma using Biuret method.

Features:

  • Method Specific To Peptide Bonds Of All Proteins
  • Liquid Stable Reagent With Long Shelf Life
  • Fast Result
  • High Linearity
  • Suitable for Fully Automated Chemistry Analyzer

Specifications

Methodology Biuret
Number Of Reagents Single
Sample Type Human Serum or Plasma
Reaction Type End Point
Reaction Direction Increasing
Blank Settings Reagent
Blank Absorbance Limit < 0.800
Primary Wavelength 546 nm
Secondary Wavelength NA
Flow Cell Temperature 37 0C
R1:R2 Mixing Ratio NA
Reagent Volume R1 1000 µL
Reagent Volume R2 NA
Sample Volume 10 µL
Standard Concentration 6 gm/dL
Incubation Time 10 Minutes
Incubation Temperature 37 0C
Unit gm/dL
Linearity 10 gm/dL
Pack Sizes 200 ml –(2×100)
Kit components Reagent, Standard Vial., Instructions for Use
Shelf life 24 months
Storage Temperature 2-8 0C