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Cholinesterase

Cholinesterase

Intended Use:

Test for quantitative estimation of Cholinesterase activity in human serum and plasma.

Introduction:

There are two forms of cholinesterase; acetyl cholinesterase and cholinesterase which is also commonly referred to as psuedocholinesterase. Acetylcholinesterase is found predominantly in erythrocytes. Cholinesterase is synthesised in the liver and is present in plasma and is the form of the enzyme routinely measured. Cholinesterase is most commonly measured as an indicator of exposure to anticholinesterases (organophosphates, including many insecticides), or inherited abnormal variants of the enzyme, which cause a decreased level of plasma cholinesterase. Increased levels of activity may be present in nephrotic syndrome or in the recovery from liver damage. Clinical diagnosis should not be made on a single test result; it should integrate clinical and other laboratory data.

Features:

  • Method Specific for Cholinesterase
  • Liquid Stable Reagent With Long Shelf Life
  • Fast Result
  • High Linearity

Specifications

Methodology Butyrylthiocoline Potassium Hexacynoferrate
Number Of Reagents Two
Sample Type Human Serum or Plasma
Reaction Type Kinetic
Reaction Direction Decreasing
Blank Settings Water
Primary Wavelength 405 nm
Secondary Wavelength NA
Flow Cell Temperature 37 0C
R1:R2 Mixing Ratio 5:1
Reagent Volume R1 500 µL
Reagent Volume R2 100 µL
Sample Volume 50 µL
Delay Time 10 Seconds
Measuring Time 90 Seconds
Factor 15000
Incubation Temperature 37 0C
Unit IU/L
Linearity 15000 IU/L
Pack Sizes 12 ml –(1×10, 1×2),24 ml (2×10, 2×2).
Kit components ReagentR1,R2, Instructions for Use
Shelf life 24 months
Storage Temperature 2-8 0C