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ALT (SGPT)

ALT (SGPT)

Intended Use:

Test for estimation of ALT SGPT activity in serum / plasma using UV –Kinetic method.

Introduction:

Elevated serum SGPT (ALT) levels are found in hepatitis, cirrhosis, and obstructive jaundice. Levels of SGPT are only slightly elevated in patients following a myocardial infarction. Characteristically SGPT is generally higher than SGOT in acute viral or toxic hepatitis whereas for most patients with chronic hepatic disease SGPT levels are generally lower than SGOT levels. ALT (SGPT) is a reagent set for determination of SGPT enzyme activity in human serum / plasma based on UV Kinetic method, recommended by International Federation of Clinical Chemistry (IFCC).

Features:

  • Method Specific To SGOT Using Aspartate Substrate
  • Liquid Stable Reagents With Long Shelf Life
  • Fast Result
  • High Linearity
  • Suitable For Fully Automated Chemistry Analyzer

Specifications

100 ml – (4×20 ml + 4×5 ml)
Methodology Kinetic (IFCC recommended)
Number Of Reagents Two
Sample Type Human Serum or Plasma
Reaction Type Kinetic
Reaction Direction Decreasing
Blank Settings Water
Blank Absorbance Limit NA
Primary Wavelength 340 nm
Secondary Wavelength NA
Flow Cell Temperature 37 0C
R1:R2 Mixing Ratio 4:1
Reagent Volume R1 800 µL
Reagent Volume R2 200 µL
Sample Volume 100 µL
Delay Time 60 Seconds
Measuring Time 30 Seconds
Factor 1746
Incubation Temperature 37 0C
Linearity 1700 IU/L
Pack Sizes 50 ml –(2×20 ml +2x5 ml ),
Kit components Reagent bottles, (R1 &R2), Instructions for Use
Shelf life 24 months
Storage Temperature 2-8 0C