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ALKALINE PHOSPHATASE

ALKALINE PHOSPHATASE

Intended Use:

Test for estimation of Alkaline Phosphatase activity in serum / plasma using PNPP method.

Introduction:

Serum Alkaline Phosphatase Test levels are of interest in the diagnosis of hepatobiliary disorders and bone disease associated with increased osteoblastic activity. Moderate elevations of Alkaline Phosphatase may be seen in Hodgkin’s disease, congestive heart failure, ulcerative colitis, regional enteritis, and intra-abdominal bacterial infections. Alkaline Phosphatase kitis a reagent set for determination ofAlkaline Phosphatase activity based on kinetic method using p-nitrophenylphosphate.

Features:

  • Kinetic Method Using α-Naphthyl Phosphate Substrate
  • Working Reagent Stable for One Week
  • Freedom to Test Total or Nonprostatic Acid Phosphatase
  • Fast Result
  • High Linearity
  • Two Reagents with Easy Preparation
  • Suitable for Fully Automated Chemistry Analyzer

Specifications

Methodology Kinetic (α- NapthylphosKinetic (PNPP Substrate)
Number Of Reagents Single
Sample Type Human Serum or Plasma
Reaction Type Kinetic
Reaction Direction Increasing
Blank Settings Water
Blank Absorbance Limit NA
Primary Wavelength 405 nm
Secondary Wavelength NA
Flow Cell Temperature 37 0C
R1:R2 Mixing Ratio NA
Reagent Volume R1 1000 µL
Reagent Volume R2 NA
Sample Volume 10 µL
Calibrator Concentration NA
Delay Time 60 Seconds
Measuring Time 180 Seconds
Incubation Temperature 37 0C
Linearity 2000 IU/L
Pack Sizes 25 ml –(5×5 ml), 50 ml – (10×5 ml)
Kit components Reagent bottles, Instructions for Use
Shelf life 24 months
Storage Temperature 2-8 0C