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UREASE BERTHELOT

Intended Use:

Test for estimation of Urea in serum / plasma using UREASE BERTHELOT method.

Introduction:

Urea is the end product of protein metabolism. It is synthesized in liver and transported to kidney for excretion. Increased Urea / Urea Nitrogen levels are associated with renal diseases as well as dehydration, gastrointestinal hemorrhage and diabetic coma. Decreased values are observed in some cases of severe liver diseases. Urea can be estimated in serum / plasma using Urease / Chromogen method.

Features:

Enzymatic Method Specific For Urea
Liquid Stable Reagents With Long Shelf Life
Stable Reagent Blank
Fast Result
High Linearity
Stable Standard Factor

Specifications

200ml +2x50 ml +2×50 ml)

Methodology	Urease – Berthelot
Number Of Reagents	Two
Sample Type	Human Serum or Plasma
Reaction Type	End Point
Reaction Direction	Increasing
Blank Settings	Reagent
Blank Absorbance Limit	< 0.300
Primary Wavelength	578 nm
Secondary Wavelength	NA
Flow Cell Temperature	37 0C
R1:R2 Mixing Ratio	1:1
Reagent Volume R1	500 µL
Reagent Volume R2	500 µL
Sample Volume	10 µL
Incubation Time	10 Minutes
Standard Concentration	50 mg/dl
Incubation Temperature	37 0C
Unit	mg/dl
Linearity	350 mg/dl
Pack Sizes	100 ml –(1×50 ml + 1×50 ml),
Shelf life	24 months
Storage Temperature	2-8 0C

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