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Ast (SGOT)

Ast (SGOT)

Intended Use:

Test for estimation of SGOT activity in serum / plasma using UV –Kinetic method.

Introduction:

The activity of SGOT (AST) in serum is increased significantly during heart, liver, kidney and muscle disease. The activity of SGOT (AST) is increased in 4-8 hours following a myocardial infarction, reaching its peak in 2-3 days and declining on the fourth- or fifth-day post infarction. SGOT is a reagent set for determination of GOT (AST) in human serum / plasma based on UV Kinetic method, recommended by International Federation of Clinical Chemistry (IFCC).

Features:

  • Method Specific To SGOT Using Aspartate Substrate
  • Liquid Stable Reagents With Long Shelf Life
  • Fast Result
  • High Linearity
  • Suitable For Fully Automated Chemistry Analyzer

Specifications

Methodology Kinetic (IFCC recommended)
Number Of Reagents Two
Sample Type Human Serum or Plasma
Reaction Type Kinetic
Reaction Direction Decreasing
Blank Settings Water
Blank Absorbance Limit NA
Primary Wavelength 340 nm
Secondary Wavelength NA
Flow Cell Temperature 37 0C
R1:R2 Mixing Ratio 4:1
Reagent Volume R1 800 µL
Reagent Volume R2 200 µL
Sample Volume 100 µL
Delay Time 60 Seconds
Measuring Time 30 Seconds
Factor 1746
Incubation Temperature 37 0C
Linearity 1700 IU/L
Pack Sizes 50 ml –(2×20 ml +2x5 ml ),
100 ml –(4×20 ml + 4×5 ml)
Kit components Reagent bottles, (R1 &R2), Instructions for Use
Shelf life 24 months
Storage Temperature 2-8 0C