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Adenosine Deaminase (ADA)

Adenosine Deaminase (ADA)

Intended Use:

Test for quantitative estimation of Adenosine Deaminase (ADA) activity in serum, plasma and other body fluids (Pleural Fluid, Pericardial Fluid, Peritoneal Fluid, Cerebrospinal Fluid).

Introduction:

Adenosine Deaminase (ADA) is an enzyme catalysing the deamination reaction from adenosine to inosine. The enzyme is widely distributed in human tissues, and is especially high in T lymphocytes. Elevated serum ADA activity has been observed in patients with acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis, liver cirrhosis, viral hepatitis and hepatoma Increased Adenosine Deaminase activity was also observed in patients with tuberculous effusions. Determination of ADA activity in patient serum may add unique values to the diagnosis of liver diseases in combination with ALT or γ-GT (GGT) tests. ADA assay may also be useful in the diagnosis of tuberculous pleuritis.

Features:

  • Enzymatic Method Specific for ADA
  • Liquid Stable Reagent with Long Shelf Life
  • Fast Result
  • High Linearity

Specifications

Methodology PNP-XOD
Number Of Reagents Two
Sample Type Human Serum or Plasma or Other Body Fluids
Reaction Type Kinetic
Reaction Direction Increasing
Blank Settings Water
Blank Absorbance Limit NA
Primary Wavelength 546 nm
Secondary Wavelength NA
Flow Cell Temperature 37 0C
R1:R2 Mixing Ratio 2:1
Reagent Volume R1 360 µL
Reagent Volume R2 180 µL
Sample Volume 10 µL
Calibrator Concentration On Vial IU/L
Delay Time 60 Seconds
Measuring Time 180 Seconds
Incubation Temperature 37 0C
Linearity 200 IU/L
Pack Sizes 15 ml –(1×10 ml + 1×5 ml),30 ml – (1×20 ml + 1×10 ml)
Kit components Reagent bottles(R1 &R2), Calibrator vial liquid stable , Instructions for Use
Shelf life 24 months
Storage Temperature 2-8 0C